Yeast Display Antibody Discovery
Accelerating therapeutic antibody discovery with precision and scalability
At DuneX Biosciences, we specialize in yeast display–based antibody discovery and engineering, providing end-to-end solutions for biotech and pharmaceutical partners. Our advanced FACS-based selection platform enables rapid identification, affinity maturation, and validation of high-performing antibody candidates — reducing timelines and boosting discovery success rates.
Request a QuoteWorkflow Overview
Our streamlined yeast display discovery workflow integrates advanced automation, data analytics, and high-throughput screening to deliver validated antibody candidates rapidly and efficiently.
Step 1 – Library Design
Design and construct scFv or Fab libraries (immune, naïve, or synthetic) with diversity up to 10⁹ variants.
Step 2 – Display Induction
Express antibody fragments on yeast surface and verify display quality using flow cytometry.
Step 3 – FACS Sorting
Perform multi-round FACS selection using Sony SH800 for quantitative enrichment of high-affinity binders.
Step 4 – Clone Characterization
Screen individual clones by ELISA or flow binding assays to validate specificity and signal strength.
Step 5 – NGS & Data Analysis
Perform deep sequencing and bioinformatic clustering to identify top antibody families and mutational patterns.
Step 6 – Expression & Validation
Express and purify lead antibody candidates for affinity validation, kinetic profiling, and functional assays.
Our Core Services
| Service Module | Description | Starting Price (USD) |
|---|---|---|
| Custom Library Construction | Design and generate scFv or Fab libraries (immune, semi-synthetic, or naïve, 10⁷–10⁹ diversity). | from $8,000 |
| FACS-Based Binder Selection | Multi-round enrichment on target antigens using Sony SH800 / BD FACSAria; gating based on antigen-binding fluorescence. | from $5,000 per round |
| Affinity Maturation | Directed evolution through chain shuffling or error-prone PCR to enhance affinity and specificity. | from $6,000 |
| Epitope Binning & Kinetic Screening | BLI / SPR analysis to rank clones by binding kinetics and epitope diversity. | from $3,500 |
| Sequencing & Bioinformatics | NGS-based repertoire profiling, clone clustering, and sequence optimization report. | from $2,000 |
| Recombinant Expression & Purification | Small-scale antibody expression (E. coli / mammalian) and purification for validation assays. | from $2,500 |
⏱ Turnaround: 6–10 weeks per complete discovery campaign
📦 Deliverables: Top antibody sequences + binding data + enriched yeast clones
🧫 Applications: Therapeutic antibodies • Bispecifics • Diagnostic reagents • Target validation
Package Pricing
| Package | Includes | Timeline | Package Price (USD) |
|---|---|---|---|
| Discovery Starter | 1 library (10⁷ diversity), 2 rounds FACS selection, binding validation by flow or ELISA. | 6 weeks | $18,000 – $22,000 |
| Affinity Optimization | Affinity maturation with 3 additional sorting rounds and BLI kinetic analysis for top clones. | 8 weeks | $25,000 – $32,000 |
| Full Campaign (End-to-End) | Custom library (10⁹ diversity) + up to 5 rounds FACS selection + NGS analysis + recombinant expression & purification deliverables. | 10 weeks | $45,000 – $55,000 |
💬 Custom quotations available for multi-target or bispecific antibody programs.
Why DuneX Biosciences
- Integrated Platform: From library design to clone sequencing — all performed in-house for maximum data consistency.
- High-Diversity Libraries: Up to 10⁹ unique variants enable robust binder discovery and affinity optimization.
- Automated FACS Precision: Sony SH800-based sorting ensures reproducible selection and quantitative enrichment.
- Rapid Turnaround: 6 – 10 weeks typical from target to validated antibody candidates.
- Expert Scientific Team: Deep experience in CRISPR, yeast display, and antibody engineering across oncology and immunology programs.
- Data Transparency: All flow plots, gating strategies, and NGS sequence data delivered with full documentation.