Up to ~10–15 kb cargo capacity
Streamlined plasmid-only workflow
Why Sleeping Beauty?
Sleeping Beauty (SB) transposon–based integration offers a powerful alternative to viral vectors. For many research applications, SB enables simpler logistics, larger genetic payloads, and faster project execution — without the need to produce high-titer viral stocks.
Plasmid-only workflow, no viral production.
Reduced biosafety and logistics burden compared to lentivirus or AAV.
Non Viral and Straightforward
Efficient integration of large transgenes, multi-cistronic cassettes, or genetic circuits.
Ideal when your construct exceeds typical lentiviral size limits.
High cargo capacity
No viral packaging lead time or QC.
Faster “design → construct → stable line” cycle at competitive cost.
Cost- and time-efficient
Applications
Our Sleeping Beauty platform is optimized for research teams who need reproducible, integration-ready cell models without the overhead of viral production.
CAR / TCR and receptor engineering
Stable expression of CARs, TCRs, or synthetic receptors for functional assays and optimization.
Reporter and sensor cell lines
Integration of fluorescent or luminescent reporters, biosensors, or CRISPR activity readouts.
Large transgene and circuit expression
Overexpression of large cDNAs, polycistronic cassettes, or genetic circuits that challenge viral packaging limits.
Stable expression for screening & assay development
Building robust assay cell lines prior to CRISPR screens, drug response profiling, or antibody testing.
Workflow Overview
We handle the complete process — from construct design through assay-ready, cryopreserved vials.
Project design & vector strategy
We review your target(s), construct design, and cell line requirements, then recommend an SB vector configuration, selection marker, and transposase format (DNA or mRNA).
Plasmid construction & QC
We clone your gene(s) or cassette into a Sleeping Beauty transposon backbone and perform sequence-verified QC.
Transfection & transposition
Your chosen parental cell line is co-transfected with SB transposon and transposase. We optimize conditions to balance integration efficiency and cell health.
Selection & enrichment
Antibiotic selection and/or fluorescence-based enrichment (if applicable) are used to establish stable, bulk populations.
Clonal isolation (optional)
For applications requiring defined integration profiles or uniform expression, we isolate single-cell clones and expand multiple candidates.
Characterization & deliverables
We confirm transgene expression and basic phenotype (per agreed-upon assays), then provide validated cell vials, sequence maps, and a concise data report.
Service Packages
Pilot Stable Pool for <5kb inserts
From $1,999
Sleeping Beauty construct design & cloning (1 cassette)
Transfection & antibiotic selection
Bulk stable pool (no clonal isolation)
Basic expression QC (qRT-PCR)
2 weeks TAT
Custom / Complex Constructs
From $3,999
Support for large (Up to 15 kb) or multi-gene constructs
4 weeks TAT
Assay-Ready Clonal Line
From $4,999
Single-cell cloning (e.g., limiting dilution / FACS)
4–8 candidate clones screened
1–2 lead clones fully expanded and banked
Extended expression QC (fluorescence / Western blot / flow cytometry)
Note: Cloning service is an extra charge. All services are for research use only and not for use in humans or clinical applications.
