Protocols
Simplifying your path from project idea to scientific results.
CRISPR Functional Genomics Protocols
Lentiviral Production & Titration
MOI Optimization Protocol
Genome-Wide / CRISPRi / CRISPRa Screening Protocols
Genomic DNA Extraction
Two-Step PCR Amplification
MAGeCK-Based Analysis
Sleeping Beauty Stable Cell Line Engineering
SB Transposon System: Transfection & Integration
SB Antibiotic Selection & Enrichment
Stable Cell Pool QC & Expression Validation
Single-Cell Cloning Protocol
Transgene Copy Number & Integration Site Analysis
Cell Banking SOP
Yeast Display Antibody Discovery
Yeast Surface Display Library Construction
Yeast Transformation & Library Electroporation
Antigen Labeling & Binding Assay
FACS-Based Enrichment
Affinity Maturation via Error-Prone PCR
Plasmid Rescue & Candidate Sequencing
NGS Sample Preparation Protocols
Amplicon Library Construction
Library QC Protocol
Illumina Sequencing Run Setup
Cell Culture & Analytical Core Protocols
Mammalian Cell Culture Maintenance
Cryopreservation & Thawing SOP
DNA/RNA Extraction & Purification
High-Speed Centrifugation & Sample Prep
Flow Cytometry Acquisition & Analysis
