CRISPR Functional Genomics Screening
Uncover novel drug targets and resistance mechanisms with genome-scale precision
DuneX Biosciences provides comprehensive CRISPR screening services to identify gene dependencies, synthetic lethal interactions, and phenotypic drivers of drug response. Our platform integrates genome-wide knockout or activation libraries with high-content readouts and next-generation sequencing.
Our Screening Routes
Drug Synthetic Lethal Screen
Identify genes whose loss enhances or suppresses sensitivity to a specific therapeutic compound. This route is ideal for target identification, mechanism-of-action elucidation, and drug combination discovery.
- Genome-wide or custom sgRNA library (10⁵–10⁶ guides)
- Drug-treated vs. control cell populations
- NGS-based dropout or enrichment analysis (MAGeCK / CRISPResso2)
- Hit prioritization and pathway enrichment mapping
💡 Deliverables: ranked gene lists, volcano plots, pathway analyses, and validation-ready hit data.
FACS-Based Phenotypic Screen
Discover genetic drivers of surface marker expression, signaling activity, or cell fate using fluorescence-activated cell sorting (FACS) as the selection readout. Ideal for immunology, oncology, and cell therapy programs.
- CRISPR knockout / activation libraries in mammalian cells
- Sorting based on fluorescent reporter intensity or antibody staining
- Sorted population sequencing and differential sgRNA enrichment
- Data integration with single-cell expression or pathway analysis
🔬 Deliverables: FACS gating data, differential gene lists, and pathway enrichment reports.
Package Pricing
Drug Synthetic Lethal Screen
| Service | Description | Timeline | Price (USD) |
|---|---|---|---|
| Full Synthetic Lethal Screen | Genome-wide or focused loss-of-function screen comparing drug-treated and control populations. Includes transduction, treatment, NGS, and analysis report (MAGeCK / DESeq2). | 6–8 weeks | $15 000 |
| Library Preparation & Sequencing Only | gDNA extraction, PCR amplification of sgRNA regions, Illumina sequencing, and read count matrix generation. | 1–2 weeks | $3 000 / sample |
| CRISPR Library Construction | Custom pooled library cloning (genome-wide or targeted); QC by NGS and representation metrics. | 3–4 weeks | $4 000 |
FACS-Based Phenotypic Screen
| Service | Description | Timeline | Price (USD) |
|---|---|---|---|
| Full FACS-Based Screen | CRISPR knockout or activation library screen using FACS selection on fluorescent reporters or surface-marker staining. Includes sorting, sequencing, and analysis. | 6–8 weeks | $17 000 |
| Library Preparation & Sequencing Only | Processing of sorted vs unsorted cell populations; sgRNA sequencing and enrichment analysis. | 1–2 weeks | $3 000 / sample |
| CRISPR Library Construction | Custom gRNA library design and cloning into lentiviral backbone, QC by NGS and representation profiling. | 3–4 weeks | $4 000 |
📦 Deliverables: Raw & processed NGS data, ranked gene lists, volcano plots,
and pathway-level enrichment reports
🔬 Applications: Synthetic lethality • Drug-response modifiers • Immune signaling drivers
💬 Custom quotation available for multiplexed drug or dual-cell-line projects.
Add-on Capabilities
- Custom library design (focused, genome-wide, or pathway-specific)
- Drug treatment optimization and cell-line engineering
- NGS data analysis and hit validation
- CRISPRa / CRISPRi screening for gain- or loss-of-function studies
- Secondary validation with single-guide re-tests or orthogonal assays